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mouse anti neun  (Proteintech)


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    Structured Review

    Proteintech mouse anti neun
    Mouse Anti Neun, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+neun/NeuN+Antibody/pmc13642301-92-34-38
    Average 96 stars, based on 724 article reviews
    mouse anti neun - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Protectin DX alleviates lumbar radicular pain in rats by suppressing RIP3/MLKL-associated necroinflammation through restoration of autophagic function
    Article Snippet: Cryosections (10 μm) were prepared, thoroughly rinsed with sterile PBS, and blocked with QuickBlock Buffer (Beyotime, China) for 10 min at room temperature. .. The sections were then incubated overnight at 4 °C with a primary antibody cocktail containing rabbit anti-MLKL (DF7412; 1:150; Affinity, USA), mouse anti-GFAP (GB12096; 1:500; Servicebio, China), mouse anti-Iba1 (GB12105; 1:500; Servicebio, China), and mouse anti-NeuN (66836-1-Ig; 1:100; Proteintech, USA). .. After washing with PBS, the sections were incubated in the dark at 37 °C for 60 min with DyLight 488-conjugated goat anti-rabbit IgG (1:500; Abbkine, USA) and DyLight 594-conjugated goat anti-mouse IgG (1:500; Abbkine, USA).

    Article Title: CSF pTDP-43 across the Alzheimer's spectrum: Links to amyloid, APOE and vasculopathy.
    Article Snippet: .. Chicken anti-GFAP (1:500, Merck) or mouse anti-NeuN (1:200, Proteintech) were used as primary antibodies and were together with the rabbit anti-pTDP-43 Ser409/410 incubated overnight (ON) at 4 ◦C. .. For secondary detection in the NeuN/ pTDP-43 staining, goat anti-mouse Alexa Fluor 488 (1:250, Vector Laboratories) was applied and incubated ON at 4 ◦C.

    Article Title: Manipulation therapy alleviates neuropathic pain via the SOCS1 m 6 A epigenetic loop.
    Article Snippet: .. After phosphate buffered saline (PBS) washing, sections were blocked with 10% goat serum for 1 hour at room temperature, followed by overnight incubation at 4°C with the following primary antibodies: rabbit anti-METTL3 (1:200, 15073-1-AP; Proteintech), anti-SOCS1 (1:200, AF5378; Affinity), anti-TLR4 (1:200, 66350-1-Ig; Proteintech); mouse anti-NeuN (1:200, 66836-1-Ig; Proteintech), anti-GFAP (1:200, 60190-1-Ig; PCR: polymerase chain reaction, SOCS1: suppressor of cytokine signaling 1 protein, TLR4: toll-like receptor 4, FTO: fat mass and obesityassociated protein. .. Liping Wu, et al https://doi.org/10.3344/kjp.251246 Proteintech), and anti-IBA1 (1:200, 66827-1-Ig; Proteintech).

    Article Title: Bidirectional regulation of social memory by two distinct types of GABAergic neurons in the lateral septum.
    Article Snippet: Brains were dissected and post-fixed in 4% PFA for 6 h at 4 °C, then immersed in 30% sucrose for 2 days before sectioning (30 μm) on a cryostat (Leica CM1050; Leica Biosystems, Germany). .. For IHC, slices were pre-incubated in blocking solution (10% normal goat serum with 0.3% Triton X-100 in PBS) for 1 h at room temperature (RT) and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CB (1:500, #14479-1-AP; Proteintech, China), mouse anti-NeuN (1:1 000, #MAB377; Merck, Germany), guinea pig anti-c-fos (1:1 000, #226004; SYSY, Germany), rabbit anti-RGS14 (1:1 000, #16258-1-AP; Proteintech, China), rat antimCherry (1:1 000, #M11217; Thermo Fisher Scientific, USA), and rabbit anti-GFP (1:1 000, #A-11122; Thermo Fisher Scientific). .. The slices were washed in PBS (10 min, three times) and incubated with secondary antibodies (1:1 000, goat anti-rabbit Alexa Fluor 488, goat anti-rat Alexa Fluor 546, goat antiguinea pig Alexa Fluor 633, and goat anti-mouse Alexa Fluor 647; Invitrogen, USA) for 2 h at RT.

    Article Title: Acupuncture modulates actin homeostasis and promotes myelin sheath regeneration in rat models of intracerebral hemorrhage.
    Article Snippet: 1 First Clinical Medical College, Heilongjiang University of Chinese Medicine, Harbin 150040, China 2 Third Department of Acupuncture and Moxibustion, First Affiliated Hospital of Heilongjiang University of Chinese Medicine, 26 Heping Road, Xiangfang District, Harbin 150040, China Abstract The relationship between actin dynamics and remyelination after intracerebral hemorrhage (ICH) remains unclear.. This study aims to investigate the mechanism by which scalp acupuncture (SA) regulates remyelination in acute ICH rats through the PAK1/LIMK1/CFL1 signaling pathway.. The Male Sprague-Dawley rats were used to establish the ICH model by autologous blood injection.

    Saline:

    Article Title: Manipulation therapy alleviates neuropathic pain via the SOCS1 m 6 A epigenetic loop.
    Article Snippet: .. After phosphate buffered saline (PBS) washing, sections were blocked with 10% goat serum for 1 hour at room temperature, followed by overnight incubation at 4°C with the following primary antibodies: rabbit anti-METTL3 (1:200, 15073-1-AP; Proteintech), anti-SOCS1 (1:200, AF5378; Affinity), anti-TLR4 (1:200, 66350-1-Ig; Proteintech); mouse anti-NeuN (1:200, 66836-1-Ig; Proteintech), anti-GFAP (1:200, 60190-1-Ig; PCR: polymerase chain reaction, SOCS1: suppressor of cytokine signaling 1 protein, TLR4: toll-like receptor 4, FTO: fat mass and obesityassociated protein. .. Liping Wu, et al https://doi.org/10.3344/kjp.251246 Proteintech), and anti-IBA1 (1:200, 66827-1-Ig; Proteintech).

    Polymerase Chain Reaction:

    Article Title: Manipulation therapy alleviates neuropathic pain via the SOCS1 m 6 A epigenetic loop.
    Article Snippet: .. After phosphate buffered saline (PBS) washing, sections were blocked with 10% goat serum for 1 hour at room temperature, followed by overnight incubation at 4°C with the following primary antibodies: rabbit anti-METTL3 (1:200, 15073-1-AP; Proteintech), anti-SOCS1 (1:200, AF5378; Affinity), anti-TLR4 (1:200, 66350-1-Ig; Proteintech); mouse anti-NeuN (1:200, 66836-1-Ig; Proteintech), anti-GFAP (1:200, 60190-1-Ig; PCR: polymerase chain reaction, SOCS1: suppressor of cytokine signaling 1 protein, TLR4: toll-like receptor 4, FTO: fat mass and obesityassociated protein. .. Liping Wu, et al https://doi.org/10.3344/kjp.251246 Proteintech), and anti-IBA1 (1:200, 66827-1-Ig; Proteintech).

    Immunohistochemistry:

    Article Title: Bidirectional regulation of social memory by two distinct types of GABAergic neurons in the lateral septum.
    Article Snippet: Brains were dissected and post-fixed in 4% PFA for 6 h at 4 °C, then immersed in 30% sucrose for 2 days before sectioning (30 μm) on a cryostat (Leica CM1050; Leica Biosystems, Germany). .. For IHC, slices were pre-incubated in blocking solution (10% normal goat serum with 0.3% Triton X-100 in PBS) for 1 h at room temperature (RT) and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CB (1:500, #14479-1-AP; Proteintech, China), mouse anti-NeuN (1:1 000, #MAB377; Merck, Germany), guinea pig anti-c-fos (1:1 000, #226004; SYSY, Germany), rabbit anti-RGS14 (1:1 000, #16258-1-AP; Proteintech, China), rat antimCherry (1:1 000, #M11217; Thermo Fisher Scientific, USA), and rabbit anti-GFP (1:1 000, #A-11122; Thermo Fisher Scientific). .. The slices were washed in PBS (10 min, three times) and incubated with secondary antibodies (1:1 000, goat anti-rabbit Alexa Fluor 488, goat anti-rat Alexa Fluor 546, goat antiguinea pig Alexa Fluor 633, and goat anti-mouse Alexa Fluor 647; Invitrogen, USA) for 2 h at RT.

    Blocking Assay:

    Article Title: Bidirectional regulation of social memory by two distinct types of GABAergic neurons in the lateral septum.
    Article Snippet: Brains were dissected and post-fixed in 4% PFA for 6 h at 4 °C, then immersed in 30% sucrose for 2 days before sectioning (30 μm) on a cryostat (Leica CM1050; Leica Biosystems, Germany). .. For IHC, slices were pre-incubated in blocking solution (10% normal goat serum with 0.3% Triton X-100 in PBS) for 1 h at room temperature (RT) and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CB (1:500, #14479-1-AP; Proteintech, China), mouse anti-NeuN (1:1 000, #MAB377; Merck, Germany), guinea pig anti-c-fos (1:1 000, #226004; SYSY, Germany), rabbit anti-RGS14 (1:1 000, #16258-1-AP; Proteintech, China), rat antimCherry (1:1 000, #M11217; Thermo Fisher Scientific, USA), and rabbit anti-GFP (1:1 000, #A-11122; Thermo Fisher Scientific). .. The slices were washed in PBS (10 min, three times) and incubated with secondary antibodies (1:1 000, goat anti-rabbit Alexa Fluor 488, goat anti-rat Alexa Fluor 546, goat antiguinea pig Alexa Fluor 633, and goat anti-mouse Alexa Fluor 647; Invitrogen, USA) for 2 h at RT.



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